D Y Patil International University, Akurdi, Pune

Mid exam (20)

Seminar (10)

Lab practical (30)

Final exam (40)

Total (100)

Module No.

Topics

Lectures

1.

Introduction to Downstream processing

Introduction to downstream processing, principles, characteristics of bio-molecules and bioprocesses. Cell disruption for product release – mechanical, enzymatic and chemical methods. Pre-treatment and stabilisation of bio-products.

6

2.

Physical methods of separation

Unit operations for solid-liquid separation - filtration and centrifugation.

8

3.

Isolation of products

Adsorption, liquid-liquid extraction, aqueous two-phase extraction, membrane separation – ultrafiltration and reverse osmosis, dialysis, precipitation of proteins by different methods.

8

4.

Product purification

Chromatography – principles, instruments and practice, adsorption, reverse phase, ion exchange, size exclusion, hydrophobic interaction, bio-affinity and pseudo affinity chromatographic techniques.

8

5.

Final product formulation and finishing operations

Crystallization, drying and lyophilization in final product formulation

8

Sr. No

Content (Daily Plan)

Date (Proposed)

1

Introduction to downstream processing

05/08/24

2

Characteristics of biomolecules and bioprocesses

09/08/24

3

Cell disruption- physical and chemical methods

12/08/24

4

Cell disruption- biological and mechanical methods

14/08/24

5

Group discussion on cell disruption techniques

21/08/24

6

Pre-treatment and stabilization of bioproducts

22/08/24

7

Basic downstream processing problems

23/08/24

8

Practice problems

28/08/24

9

Filtration Introduction

30/08/24

10

Filtration concept

02/09/24

11

Filtration – derivation

04/09/24

12

Filtration- problems

06/09/24

13

Centrifugation- Introduction

09/09/24

14

Types of centrifuges used

12/09/24

15

Problems in centrifugation

13/09/24

16

Adsorption- concept of isotherms

18/09/24

17

Liquid-liquid extraction- co-current, counter current, Batch and continuous

20/09/24

18

Aqueous two-phase extraction- principle

23/09/24

19

Membrane separation- ultrafiltration & microfiltration

04/10/24

20

Membrane separation- reverse osmosis

09/10/24

21

Dialysis and Electrodialysis

11/10/24

22

Protein precipitation methods

14/10/24

23

Chromatography – Introduction

15/10/24

24

Adsorption chromatography

17/10/24

25

Gel filtration chromatography

18/10/24

26

Ion-exchange chromatography

21/10/24

27

Hydrophobic interaction chromatography

23/10/24

28

Normal and Reverse phase chromatography

08/11/24

29

Bio affinity and Pseudo affinity chromatography

11/11/24

30

Group discussion

14/11/24

31

Crystallization

18/11/24

32

Student seminar

18/11/24

33

Drying and finishing operations

21/11/24

Sr. No

Content

Date

1

Centrifugation Studies During Settling Of Yeast Cells

21/08/24

2

Cell Disruption By Sonication and protein estimation

28/08/24

3

Batch Sedimentation & Flocculation

04/09/24

4

Basket Centrifuge and Cyclone separator – demo

11/09/24

5

HPLC- demo

18/09/24

6

Precipitation of proteins by salting out- Ammonium sulfate

25/09/24

7

Aqueous two-phase extraction and partition coefficient

10/10/24

8

Gel filtration chromatography

16/10/24

9

Affinity chromatography

23/10/24

10

Column Chromatography

13/11/24

11

Ion-Exchange Chromatography

13/11/24