Name of the Course: BRD 4005 Bioreactor design and analysis (4)
Course Instructor:
Name: Dr. Babuskin Srinivasan
Office Number:
Phone: +91-8807762115
Email: babuskin.srinivasan@dypiu.ac.in
Office hours: 9.30 am – 5.30 pm
Location: 516, D Y Patil International University, Pune
· Teaching Assistant: Nil
Name:
Contact:
Office Hours:
Location:
· Level (who can take this course?): B. Tech Bioengineering
· Credit: 3-1-0-4
· Evaluation:
· Breakup of evaluation: % wise
|
Mid exam (30) |
Seminar (10) |
Case study (15) |
Final exam (45) |
Total (100) |
· Maximum Number of Students: 27
Course Objectives:
The course introduces the student to design aspects of bioreactor. The course provides knowledge regarding the materials used for construction, biosensors, online control etc. At the end of this course, the student will be able to design and develop bioreactors used for specific operations
Prerequisites:
Basic mathematical skills like differentiation, integration
Basic knowledge about bioprocess
Course Syllabus:
|
Module No. |
Topics |
Lectures |
|
1. |
BIOCHEMICAL PROCESS VARIABLES – MEASUREMENTS & CONTROL Measurement & Control- Temperature, pH, pressure, dissolved oxygen, shaft power, rate of stirring, detection and prevention of foam, measurement of cells, inlet and outlet gas analysis. Mechanical fittings in a bioreactor. Software used in Bioreactors- SCADA |
9 |
|
2. |
BIOREACTOR DESIGN Design of batch bioreactors, Design of Fed-batch bioreactors, Design ofd Continuous Bioreactors; Design considerations for maintaining sterility of process streams processing equipments |
8 |
|
3. |
BIOREACTOR ANALYSIS Mass Transfer in bioreactor, Heat Transfer in Bioreactors, Rheology of fermentation broths, Heterogeneous reactions in bioprocesses, Scale-up of bioreactors- criteria & parameters |
8 |
|
4. |
NON-IDEAL REACTORS; RTD Design and Analysis of Non-ideal reactors, Reactors with nonideal mixing: Mixing times in agitated tanks, residence time distribution, models for nonideal reactors, Mixing-bioreaction interactions |
8 |
|
5. |
CASE STUDIES Case study analysis on type of bioreactors employed in food, biopharma and environmental treatment plants; Modelling & Simulation in bioreactor design and operation; Application of MATLAB in bioprocess. Artificial neural networking in Bioprocess |
8 |
Lesson Plan:
|
Sr. No |
Content (Daily Plan) |
Date (Proposed) |
|
1 |
Introduction to Bioreactor design |
08/08/24 |
|
2 |
Measurement - Temperature, pH |
08/08/24 |
|
3 |
Measurement- pressure |
13/08/24 |
|
4 |
Measurement- dissolved oxygen, shaft power |
20/08/24 |
|
5 |
Measurement - dissolved oxygen, shaft power |
20/08/24 |
|
6 |
Control mechanisms |
22/08/24 |
|
7 |
Type of controllers employed |
27/08/24 |
|
8 |
Mechanical fittings in a bioreactor |
27/08/24 |
|
9 |
Software used in bioreactors- SCADA |
29/08/24 |
|
10 |
Design of bioreactors- introduction |
29/08/24 |
|
11 |
Design of Batch bioreactors |
03/09/24 |
|
12 |
Design of Fed-batch bioreactors |
03/09/24 |
|
13 |
Design of continuous bioreactors |
05/09/24 |
|
14 |
Design considerations |
10/09/24 |
|
15 |
Practice problems- batch, fed batch |
12/09/24 |
|
16 |
Practice problems- continuous |
19/09/24 |
|
17 |
Mass Transfer in bioreactors |
24/09/24 |
|
18 |
Heat Transfer in Bioreactors |
01/10/24 |
|
19 |
Rheology of fermentation broths |
03/10/24 |
|
20 |
Heterogeneous reactions in bioprocesses, Scale-up of bioreactors- criteria & parameters |
03/10/24 |
|
21 |
Scale-up of bioreactors- criteria & parameters |
11/10/24 |
|
22 |
Non-ideal reactors – intro |
15/10/24 |
|
23 |
Residence time distribution |
22/10/24 |
|
24 |
Models of non-ideal reactors |
22/10/24 |
|
25 |
Student seminar |
07/11/24 |
|
26 |
Case study intro |
12/11/24 |
|
27 |
Group discussion |
14/11/24 |
|
28 |
Case study project presentation |
18/11/24 |
|
29 |
Case study project presentation |
18/11/24 |
|
30 |
Modelling and simulation of bioreactors |
19/11/24 |
|
31 |
Student seminar |
21/11/24 |
|
32 |
Tutorial class |
21/11/24 |
· Course Outcome:
Upon success completion of this course, the students will be able to:
Ø Students will be able to demonstrate knowledge regarding the construction of bioreactors
Ø Students gain inputs on the type of sensors and softwares used in the bioreactors
Ø The learners can be able to explain the type of bioreactors employed in various bioprocess sectors
Recommended Readings
1. Michael L. Shuler and FikretKargi, Bioprocess Engineering: Basic Concepts, 2nd Edition, 2002, Prentice Hall.
2. Octave Levenspiel, Chemical Reaction Engineering, 3rd Edition, 1999, Wiley
3. Moser, Anton, Bioprocess Technology: Kinetics and Reactors, Springer Verlag, 1988.
· Study Materials: shared in the form of ppts, eBooks
- Teacher: Mrs.Mayura Abhijit khanore
- Teacher: Dr. Babuskin Srinivasan
- Teacher: Dr Prafull Chavan
- Teacher: Shital Vijay Nehere
Name of the Course: DPR 4001 Downstream Processing (5.5)
Course Instructor:
Name: Dr. Babuskin Srinivasan
Office Number:
Phone: +91-8807762115
Email: babuskin.srinivasan@dypiu.ac.in
Office hours: 9.30 am – 5.30 pm
Location: 516, D Y Patil International University, Pune
· Teaching Assistant:
Name: Mrs. Mayura khanore
Contact: +919420904067
Office Hours: Time slots
Location: 340, D Y Patil International University, Pune
· Level (who can take this course?): B. Tech Bioengineering
· Credit: 3-1-3-5.5
· Evaluation:
· Breakup of evaluation: % wise
|
Mid exam (20) |
Seminar (10) |
Lab practical (30) |
Final exam (40) |
Total (100) |
·
· Maximum Number of Students: 27
Course Objectives:
The objective of this course is to learn about how to develop and formulate various product isolation methods related to biopharmaceuticals, clinical research and development
Prerequisites:
Basic mathematical skills like differentiation, integration
Basic knowledge about upstream processing
Course Syllabus:
|
Module No. |
Topics |
Lectures |
|
1. |
Introduction to Downstream processing Introduction to downstream processing, principles, characteristics of bio-molecules and bioprocesses. Cell disruption for product release – mechanical, enzymatic and chemical methods. Pre-treatment and stabilisation of bio-products. |
6 |
|
2. |
Physical methods of separation Unit operations for solid-liquid separation - filtration and centrifugation. |
8 |
|
3. |
Isolation of products Adsorption, liquid-liquid extraction, aqueous two-phase extraction, membrane separation – ultrafiltration and reverse osmosis, dialysis, precipitation of proteins by different methods. |
8 |
|
4. |
Product purification Chromatography – principles, instruments and practice, adsorption, reverse phase, ion exchange, size exclusion, hydrophobic interaction, bio-affinity and pseudo affinity chromatographic techniques. |
8 |
|
5. |
Final product formulation and finishing operations Crystallization, drying and lyophilization in final product formulation |
8 |
Lesson Plan:
|
Sr. No |
Content (Daily Plan) |
Date (Proposed) |
|
1 |
Introduction to downstream processing |
05/08/24 |
|
2 |
Characteristics of biomolecules and bioprocesses |
09/08/24 |
|
3 |
Cell disruption- physical and chemical methods |
12/08/24 |
|
4 |
Cell disruption- biological and mechanical methods |
14/08/24 |
|
5 |
Group discussion on cell disruption techniques |
21/08/24 |
|
6 |
Pre-treatment and stabilization of bioproducts |
22/08/24 |
|
7 |
Basic downstream processing problems |
23/08/24 |
|
8 |
Practice problems |
28/08/24 |
|
9 |
Filtration Introduction |
30/08/24 |
|
10 |
Filtration concept |
02/09/24 |
|
11 |
Filtration – derivation |
04/09/24 |
|
12 |
Filtration- problems |
06/09/24 |
|
13 |
Centrifugation- Introduction |
09/09/24 |
|
14 |
Types of centrifuges used |
12/09/24 |
|
15 |
Problems in centrifugation |
13/09/24 |
|
16 |
Adsorption- concept of isotherms |
18/09/24 |
|
17 |
Liquid-liquid extraction- co-current, counter current, Batch and continuous |
20/09/24 |
|
18 |
Aqueous two-phase extraction- principle |
23/09/24 |
|
19 |
Membrane separation- ultrafiltration & microfiltration |
04/10/24 |
|
20 |
Membrane separation- reverse osmosis |
09/10/24 |
|
21 |
Dialysis and Electrodialysis |
11/10/24 |
|
22 |
Protein precipitation methods |
14/10/24 |
|
23 |
Chromatography – Introduction |
15/10/24 |
|
24 |
Adsorption chromatography |
17/10/24 |
|
25 |
Gel filtration chromatography |
18/10/24 |
|
26 |
Ion-exchange chromatography |
21/10/24 |
|
27 |
Hydrophobic interaction chromatography |
23/10/24 |
|
28 |
Normal and Reverse phase chromatography |
08/11/24 |
|
29 |
Bio affinity and Pseudo affinity chromatography |
11/11/24 |
|
30 |
Group discussion |
14/11/24 |
|
31 |
Crystallization |
18/11/24 |
|
32 |
Student seminar |
18/11/24 |
|
33 |
Drying and finishing operations |
21/11/24 |
Laboratory
|
Sr. No |
Content |
Date |
|
1 |
Centrifugation Studies During Settling Of Yeast Cells |
21/08/24 |
|
2 |
Cell Disruption By Sonication and protein estimation |
28/08/24 |
|
3 |
Batch Sedimentation & Flocculation |
04/09/24 |
|
4 |
Basket Centrifuge and Cyclone separator – demo |
11/09/24 |
|
5 |
HPLC- demo |
18/09/24 |
|
6 |
Precipitation of proteins by salting out- Ammonium sulfate |
25/09/24 |
|
7 |
Aqueous two-phase extraction and partition coefficient |
10/10/24 |
|
8 |
Gel filtration chromatography |
16/10/24 |
|
9 |
Affinity chromatography |
23/10/24 |
|
10 |
Column Chromatography |
13/11/24 |
|
11 |
Ion-Exchange Chromatography |
13/11/24 |
· Course Outcome:
Upon success completion of this course, the students will be able to:
· Define the fundamentals of downstream processing for product recovery
· Understand the requirements for successful operations of downstream processing
· Describe the components of downstream equipment and explain the purpose of each
· Apply principles of various unit operations used in downstream processing and enhance problem solving techniques
· Text Book:
1. Belter, P.A., E.L. Cussler and Wei-Houhu “Bioseparations – Downstream Processing for Biotechnology”, John Wiley, 1988.
2. Nooralabettu Krishna Prasad., Downstream Process Technology: A New Horizon in Biotechnology, Prentice Hall India,2010.
3. Sivasankar, B. “Bioseparations: Principles and Techniques”. PHI, 2006.
· Reference Book:
1. Raja Ghosh “Principles of Bioseparations Engineering”. World Scientific, 2006
2. Michael R.Ladisch Bioseparations Engineering: Principles, practice and Economics, WileyInterscience,1st Edition,2001.
3. Product Recovery in Bioprocess Technology”. (BIOTOL – Biotechnology by Open Learning Series). Butterworth – Heinmann / Elsevier, 2004.
· Study Materials: shared in the form of ppts, eBooks
- Teacher: Mrs.Mayura Abhijit khanore
- Teacher: Dr. Babuskin Srinivasan