D Y Patil International University, Akurdi, Pune

Module No.

Topics

Lectures

1.

Module 1: Overview of fermentation process & Media design

Overview of fermentation industry-general requirements of fermentation process-basic configuration of fermentor (CSTR) and ancillaries, main parameters to be monitored and controlled in fermentation processes.

Criteria for good medium, medium requirements for fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements, medium formulation of optimal growth and product formation, examples of simple and complex media, design of various commercial media for industrial fermentations – medium optimization methods

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Module 2:

METABOLIC STOICHIOMETRY AND ENERGETICS Stoichiometry of cell growth and product formation, elemental balances, degrees of reduction of substrate and biomass, available electron balances, yield coefficients of biomass and product formation, maintenance coefficients energetic analysis of microbial growth and product formation, oxygen consumption and heat evolution in aerobic cultures, thermodynamic efficiency of growth.

STERILIZATION KINETICS

Thermal death kinetics of microorganisms, batch and continuous heat sterilization of liquid media, filter sterilization of liquid media, air sterilization and design of sterilization equipment - batch and continuous

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3.

Module 3: Configuration of Bioreactors & Growth kinetics

 Bioreactor designs; Types of fermentation and fermenters; Concepts of basic modes of fermentation – Batch, fed batch ad continuous; Conventional fermentation v/s biotransformation; Solid substrate, surface and submerged fermentation; two stage cultivation Packed bed reactor, airlift reactor, introduction to fluidized bed reactor bubble column reactors

Simple unstructured models for microbial growth, Monod model, growth of filamentous organisms, product formation kinetics - Leudeking- Piret models, substrate and product inhibition on cell growth and product formation. Biomass estimation – Direct and Indirect methods.

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4

Module 4: BIOREACTOR SCALE UP

Regime analysis of bioreactor processes, oxygen mass transfer in bioreactors – microbial oxygen demands; methods for the determination of mass transfer coefficients; mass transfer correlations. Scale up criteria for bioreactors based on oxygen transfer, power consumption and impeller tip speed.

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5.

Module 5:

MODELLING AND SIMULATION OF BIOPROCESSES

Study of structured models for analysis of various bioprocess – compartmental models, models of cellular energetics and metabolism, single cell models, plasmid replication and plasmid stability model. Dynamic simulation of batch, fed batch, steady and transient culture metabolism.

RECOMBINANT CELL CULTIVATION

Different host vector system for recombinant cell cultivation strategies and advantages. E.coli, yeast Pichia pastoris/ Saccharomyces cereviseae, Animal cell cultivation, plant cell cultivation, Insect cell cultivation. High cell density cultivation, process strategies, reactor considerations in the above system

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