- Teacher: Mrs.Mayura Abhijit khanore
- Teacher: Dr. Sidhartha Singh
- Teacher: Ms. Saher Dabir Pathan
- Teacher: Dr. Surabhi Sonam
- Teacher: Dr. Vijayalakshmi Srinivasan
Name of the Course: MMB 4003 Medical Microbiology Theory
- Course Instructor:
Name: Prof. Manoj Kumar AS
Office Number: 352
Phone: 9967181502
Email: manoj.kumar@dypiu.ac.in
Office hours: 9 am to 5 pm
Location: Akurdi
- Teaching Assistant:
None
- Level (who can take this course?): Graduates in Bioengineering and Biotechnology
- Credit:
L T P C
3 0 0 3
- Evaluation: Based on attendance, interactiveness, mid term examination score and end of semester examination score
- Breakup of evaluation:
Total marks: 100
Attendance & Interactiveness: 20
Mid term Exam: 30
End of Semester Exam: 50
- Maximum Number of Students: 30
- Actual Number of Students: 22
Prerequisite: Must be a student of M.Sc Medical Biotechnology
Course Syllabus:
|
COURSE CONTENTS |
LECTURES (Hrs.) |
|
Unit I: Host Pathogen Interaction: History of infectious disease, Introduction to human pathogens, Host pathogen interaction, Introduction to normal flora, Distribution and significance of normal human microbial flora (Skin, mouth, upper respiratory tract, eye, etc.). Normal flora as pathogen, accidental pathogens, oncogenic viruses. Definitions - Infection, Invasion, Pathogen, Pathogenicity, Virulence, Toxigenicity, Carriers and their types, Nosocomial infections. Modes of Transmission of infection. |
8 |
|
Unit II: Study of following groups of microbial pathogens (Morphological characters, pathogenesis, diagnosis, epidemiology, prophylaxis and treatment) Bacterial- Enteric pathogens (Salmonella), Pyogenic organisms (Staphylococcus); Viral- HIV, Dengue; Fungal-Candida; Parasite- Plasmodium & Entamoeba. |
8 |
|
Unit III: Diagnostic Methodology: General principles of diagnostic microbiology; Collection, transport and processing of clinical samples. Cultural, biochemical, serological and molecular methods for microbial typing. |
8 |
|
Unit IV: Immunology & Epidemiology: Response of immune system in specific disease. Epidemiological principles in prevention and control of diseases. Concepts of mortality/ morbidity rates, incidence and prevalence; Emerging and re-emerging diseases: examples of model bacterial, viral, fungal, and parasite diseases. |
8 |
Lesson Plan (Class room lecture):
|
Sr. No |
Content |
Date of Lecture |
|
1 |
History of Medical Microbiology and Infectious disease; Introduction to normal flora, Distribution and significance of normal human microbial flora (Skin, mouth, upper respiratory tract, eye, etc.) |
August 10, 2024 |
|
2 |
Introduction to human pathogens, Host pathogen interaction, Definitions - Infection, Invasion, Pathogen, Pathogenicity, Virulence, Toxigenicity, Carriers and their types, Modes of Transmission of infection. Nosocomial infections. Normal flora as pathogen, accidental pathogens |
August 20, 2024 |
|
3 |
Bacterial pathogens - Morphological characteristics, pathogenesis, diagnosis, epidemiology, prophylaxis and treatment of Enteric bacteria (eg. Salmonella), and Pyrogenic bacteria (eg. Staphylococcus). Other common bacterial pathogens will be covered as student assignments |
August 31, 2024 |
|
4 |
Viral pathogens - Morphological characteristics, pathogenesis, diagnosis, epidemiology, prophylaxis and treatment of HIV and Dengue viruses. Other common viral pathogens will be covered as student assignments |
September 10, 2024 |
|
5 |
Fungal pathogens - Morphological characteristics, pathogenesis, diagnosis, epidemiology, prophylaxis and treatment of Candida. Other common fungal pathogens will be covered as student assignments |
September 20, 2024 |
|
6 |
Parasites - Morphological characteristics, pathogenesis, diagnosis, epidemiology, prophylaxis and treatment of Entamoeba and Plasmodium. Other common parasites will be covered as student assignments |
September 30, 2024 |
|
7 |
Introduction to Oncogenic viruses; Oncogenic DNA viruses – EBV, HPV and HBV |
October 15, 2024 |
|
8 |
Oncogenic RNA viruses – HCV, HIV and HHV-8 |
October 30, 2024 |
|
9 |
General principles of diagnostic microbiology; Collection, transport and processing of clinical samples. Cultural, biochemical, serological and molecular methods for microbial typing |
November 15, 2024 |
|
10 |
Response of immune system in specific disease. Epidemiological principles in prevention and control of diseases. Concepts of mortality/ morbidity rates, incidence and prevalence; Emerging and re-emerging infectious diseases |
November 30, 2024 |
- References:
Kayser F. H., Bienz K. A., Eckert J. and Zinkernagel R. M. (2004), Medical Microbiology, Thieme.
- Jawetz, Melnick, &Adelberg's Medical Microbiology by Carroll KC, Hobdon JA, Miller S, Morse SA, Mietzner TA. 27th edition. Lange Publication, 2016.
- Brooks GF, Carroll KC, Butel JS and Morse SA. Jawetz, Melnick and Adelberg’sMedical Microbiology. 24th edition. McGraw Hill Publication. (2007). 3. Goering R, Dockrell H, Zuckerman M and Wakelin D. Mims Medical microbiology. 4th edition. Elsevier. (2007).
- Course Outcome:
The course will introduce students to various topics like pathogens, interaction of hosts with pathogens, normal flora and their significance. The course will emphasize on learning diagnostic methods in identification of disease-causing agents and give ideas on immunological and epidemiological aspects in control of disease.
Name of the Course: MMB 4008 Medical Microbiology Lab
- Course Instructor:
Name: Prof. Manoj Kumar AS
Office Number: 352
Phone: 9967181502
Email: manoj.kumar@dypiu.ac.in
Office hours: 9 am to 5 pm
Location: Akurdi
- Teaching Assistant:
Name: Presida Basil Vanjare
Contact: 8446001901
Office Hours: 9 am to 5 pm
Location: Akurdi
- Level (who can take this course?): Graduates in Bioengineering and Biotechnology
- Credit:
L T P C
0 0 4 2
- Evaluation: Based on Lab record, Lab experiment, Attendance and Viva
- Breakup of evaluation:
Total marks: 30
Lab record: 10 marks = 33%
Lab experiment: 10 marks = 33%
Attendance: 5 marks = 17%
Viva: 5 marks = 17%
- Maximum Number of Students: 30
- Actual Number of Students: 22
List of Practicals:
- Preparation of Nutrient broth and Nutrient Agar plates; Inoculation and culturing of bacteria in Nutrient broth, Spread plating and streaking of bacteria on agar plates; Isolation of single colonies of bacteria on Nutrient Agar plates
- Preparation and storage of bacterial glycerol stocks; Revival of bacteria from glycerol stocks
- Isolation, observation and identification of normal microbial flora of the human body (from hand and saliva).
- IMViC Test
- Widal
test
- Characterization of microorganisms by Gram staining
- Determination of minimal inhibitory concentration (MIC) of an antibiotic.
- Identification of Etiological Agent. Sequence analysis of Etiological agents using NCBI Genbank database and NCBI BLAST
Evaluation:
|
Lab Attendance |
Record book |
End term Lab experiment |
Viva |
Total |
|
5 marks |
10 marks |
10 marks |
5 marks |
30 marks |
Prerequisites: Must be a student of M.Sc Medical Biotechnology
Course Syllabus:
Lesson Plan (Laboratory):
|
Sr. No |
Content |
Date of Practical lesson |
|
1 |
Introduction to Microbiology laboratory practices |
August 2024 |
|
2 |
Preparation of Nutrient broth and Nutrient Agar plates; Inoculation and culturing of bacteria in Nutrient broth, Spread plating and streaking of bacteria on agar plates; Isolation of single colonies of bacteria on Nutrient Agar plates.
|
August 2024 |
|
3 |
Preparation and storage of bacterial glycerol stocks; Revival of bacteria from glycerol stocks
|
August 2024 |
|
4 |
Isolation, observation and identification of normal microbial flora of the human body (from hand and saliva).
|
August 2024 |
|
5 |
Characterization of microorganisms by Gram staining
|
September 2024 |
|
6 |
IMViC Test
|
September 2024 |
|
7 |
Widal test
|
October 2024 |
|
8 |
Antimicrobial testing (MIC) of an antibiotic.
|
November 2024 |
|
9 |
Identification of Etiological Agent. Sequence analysis of Etiological agents using NCBI Genbank database and NCBI BLAST
|
November 2024 |
- References:
- Kayser F. H., Bienz K. A., Eckert J. and Zinkernagel R. M. (2004), Medical Microbiology, Thieme.
- Jawetz, Melnick, &Adelberg's Medical Microbiology by Carroll KC, Hobdon JA, Miller S, Morse SA, Mietzner TA. 27th edition. Lange Publication, 2016.
- Brooks GF, Carroll KC, Butel JS and Morse SA. Jawetz, Melnick and Adelberg’sMedical Microbiology. 24th edition. McGraw Hill Publication. (2007). 3. Goering R, Dockrell H, Zuckerman M and Wakelin D. Mims Medical microbiology. 4th edition. Elsevier. (2007).
- Course Outcome:
This course is expected to create an advanced practical understanding and applied knowledge of the practice of clinical microbiology. It will combine the biochemical and pathophysiological understanding by combining clinical biochemistry with medical microbiology experiments, which quips students with the knowledge of diagnosing and treating bacterial diseases.
- Teacher: Dr Manoj Kumar
- Teacher: Dr Anu Dandona
- Teacher: Mrs Reema C Deshmukh